General Stability | Organism |
---|---|
instable in solutions of low ionic strength, stabilization by ammonium sulfate | Sus scrofa |
instable in solutions of low ionic strength, stabilization by ammonium sulfate | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
D,L-isocitrate | isozyme 2 | Zea mays | |
0.003 | - |
NADP+ | isozyme 2 | Zea mays | |
0.0031 | - |
D,L-isocitrate | isozyme 1 | Zea mays | |
0.0063 | - |
NADP+ | isozyme 1 | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Sus scrofa | 5739 | - |
mitochondrion | - |
Geobacillus stearothermophilus | 5739 | - |
mitochondrion | - |
Zea mays | 5739 | - |
mitochondrion | - |
Azotobacter sp. | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
68000 | - |
gel filtration | Sus scrofa |
81000 | 83000 | gel filtration | Zea mays |
105000 | - |
gel filtration | Cereibacter sphaeroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Sus scrofa | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
isocitrate + NADP+ | Geobacillus stearothermophilus | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
isocitrate + NADP+ | Cereibacter sphaeroides | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
isocitrate + NADP+ | Zea mays | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
isocitrate + NADP+ | Azotobacter sp. | - |
2-oxoglutarate + NADPH + H+ + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter sp. | - |
- |
- |
Cereibacter sphaeroides | - |
i.e. Rhodobacter sphaeroides | - |
Geobacillus stearothermophilus | - |
- |
- |
Sus scrofa | - |
pig | - |
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Zea mays |
Renatured (Comment) | Organism |
---|---|
overnight dialysis against guanidine-HCl, GDP buffer | Sus scrofa |
overnight dialysis against guanidine-HCl, GDP buffer | Geobacillus stearothermophilus |
overnight dialysis against guanidine-HCl, GDP buffer | Cereibacter sphaeroides |
overnight dialysis against guanidine-HCl, GDP buffer | Zea mays |
overnight dialysis against guanidine-HCl, GDP buffer | Azotobacter sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Sus scrofa | - |
seed | - |
Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
33.5 | - |
- |
Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Sus scrofa | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
isocitrate + NADP+ | - |
Geobacillus stearothermophilus | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
isocitrate + NADP+ | - |
Cereibacter sphaeroides | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
isocitrate + NADP+ | - |
Zea mays | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? | |
isocitrate + NADP+ | - |
Azotobacter sp. | 2-oxoglutarate + NADPH + H+ + CO2 | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Zea mays |